Improvement of Bacillus clausii cell HH1 density via culture medium optimization and pH-stat fed batch fermentation

In this study, the culture medium and fermentation modes were studied aiming to improve cell density of Bacillus clausii. Firstly, factorial design method using Minimum Run Resolution IV was used evaluate relative importance components growth The results showed that three peptone, yeast extract malt significantly affecting bacterial biomass. Then, optimization these ingredient concentrations a response surface methodology with Box-Behnken resulted maximal biomass had been achieved containing 7.64 g/L 10 6.36 extract. Finally, pH-stat fed batch conducted in 2-liter bioreactor where 9X concentrated optimal feed into based on pH signal. As result, microbial increased by 2.9-fold compared through fermentation.